Biotin cy5
WebCy5.5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly … WebCy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly …
Biotin cy5
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WebAdd biotinylated secondary antibody (select Format - Biotin) at the recommended dilution (see specific datasheet for details). Incubate for at least 30 minutes at room temperature. Wash 3 times in TBS. Add enzyme-conjugated streptavidin at the recommended dilution (see specific datasheet for details). WebA biotin modified Cy5 dye. 1mg. This product is currently out of stock and unavailable. Biotin labelled, near infrared fluorescent Cy5 dye derivative that has a maximum …
WebAug 31, 2024 · A 5 μL aliquot of Cy5-double biotin DNA linkage and Cy5-Ab complexes were dropped on a carbon grid for 30 min at RT and blotted with filter paper, leaving a small amount of the solution on the carbon grid surface. Then, a 5 μL ammoniacal Ag solution of 10% NH 4 OH with 0.1 mM AgNO 3 was added and blotted with a filter paper for 10 s. WebNov 11, 2006 · To investigate the fluorescence enhancement, both SIF coated coverslips and bare glass coverslips with identical BSA-biotin/avidin layers were incubated in a solution of biotin-Cy5-labeled oligonucleotides in 10 mM HEPS buffer (pH 7) for 1 h. The dye concentration was maintained at nanomolar levels (0.2–0.5 nM).
WebMore Information. Unit Size. 1 mg. Applications. Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation. Recommended Usage. Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. Solution. WebPNA FISH probes hybridize to target DNA with high sensitivity and specificity mainly due to the electrical neutrality of its chemical structure. This trait enables PNA probes to be more efficient and useful in applying …
WebBiocytin is a chemical compound that is an amide formed from the vitamin biotin and the amino acid L -lysine. As an intermediate in the metabolism of biotin, biocytin occurs …
WebSynonyms: Goat F(ab')2 Anti-Biotin CY5.5 Conjugated Antibody, Goat F(ab')2 Anti-Biotin Antibody CY5.5 Conjugation, Goat Fab2 Anti-Biotin Antibody CY5.5 Conjugation. Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer … fellowes ayla 125WebApr 4, 2024 · Cy5.5 (Cyanine 5.5) 是一种发近红外(NIR)荧光的花青素荧光染料。它的消光系数高，荧光也很亮，并且对pH不敏感，并且由于它的荧光波长(Em：~700nm)恰好处于肌体组织近红外窗口 I 的区域(肌体的血液，体液和组织此区域背景荧光弱，而长波长穿透性强)，所以Cy5.5常应用于小动物活体体内成像中。 fellowes b161chttp://www.delta-f.com/details/1167920 definition of gaffWeb产品简述： Cy5.5-PEG-Biotin，生物素可以很容易被检测，链霉亲和素结合法是广泛使用的分子靶点检测。修饰性PEG广泛应用于修饰蛋白类药物、肽类化合物、有机小分子药物、生物材料等。花氰染料Cyanine常被应用于生… definition of gaffedWebCy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). ... Jejunum villi superficial epithelium labeled with Ulex-biotin Streptavidin-Cy5 (016-170-084 blue), gastric K ... fellowes b151cWebThis bifunctional Cy5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high … definition of gaffeWebMolecular Formula: C 25 H 42 N 6 O 13 P 2 S 1 (free acid) Molecular Weight: 728.65 g/mol (free acid) Exact Mass: 728.20 g/mol (free acid) Purity: ≥ 95 % (HPLC) Form: solution in 10 mM Tris-HCl Color: colorless … definition of gadget