Chlamydomonas stock center
WebJul 1, 2014 · C. reinhardtii strain cw10 (cc849, mating type minus) was obtained from the Chlamydomonas Stock Center, Duke University, Durham, NC. All cells were maintained on agar plates containing minimal medium [19] and were kept in high-CO 2 (~ 5% CO 2 in air) growth chambers at room temperature. WebJun 9, 2000 · The cell wall-less strain cc840 (mt − cw10; Chlamydomonas stock center, Duke University) was crossed to thepsaBΔ strain, and cw10 psaBΔ segregants were selected and verified by phenotypic and polymerase chain reaction analysis. Cell Breaking, Chloroplasts, and Thylakoids Isolation
Chlamydomonas stock center
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WebApr 11, 2024 · Glycerolipid responses to naphthenic acid exposure were explored in two green algae. • Chlorella pyrenoidosa was more tolerant to CHCA than Chlamydomonas reinhardtii.. Growth, photosynthesis and morphology of C. pyrenoidosa were not affected by CHCA.. Two algae both remodeled glycerolipid components for acclimation to CHCA … WebDownload scientific diagram Kinetic parameters for 3PGA and P i inhibition of ADPglucose pyrophosphorylase from wild-type and mutant strains (pyrophosphorolysis direction). A, display of the P i ...
WebDec 1, 1999 · Growth Conditions and Strains. CC125 (Chlamydomonas stock center, Duke University, Durham, NC) was the wild-type strain and the psr1–1 and psr1–2 mutants were outcrossed to CC125 at least four times to ensure genetic homogeneity.cw15 and nit1 mutations were introduced into the genetic backgrounds to increase the efficiency of and … WebApr 17, 2001 · Wild-type CC-125 originally was obtained from the Chlamydomonas Stock Center, Duke University, Durham, NC. The cell wall-deficient, high CO 2-requiring cia5 double mutant was obtained from James Moroney (Louisiana State University, Baton Rouge) and was maintained in Tris acetate phosphate (TAP) medium ().Liquid cultures …
WebJan 13, 2024 · Many axonemal proteins enter cilia and flagella on intraflagellar transport (IFT) trains, which move bidirectionally along the axonemal microtubules. Certain axonemal substructures including the radial spokes and outer dynein arms are preassembled in the cell body and transported as multisubunit complexes into flagella by IFT. Here, we used … WebMar 24, 2024 · The wild-type C. reinhardtii strain CC-124 was obtained from the Chlamydomonas Stock Center (Duke . University, Durham, NC, USA). 2.3. Stable transformation of C. reinhardtii.
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WebJan 1, 2024 · Chlamydomonas • Abstract Cell-surface display systems are biotechnological techniques used to express on the cell surface. Their application depends directly on the … northeastern family portal loginWebThe Chlamydomonas Stock Center has a HSM recipe based off of Beijerink’s salt stocks. NH4Cl Stock Solution (1L) Bring the volume to 1 L with MQ-H 2 O Autoclave Ca/Mg … northeastern fall transfer decision dateWebMar 31, 2016 · View Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn … northeastern fall 2023 calendarWebAug 16, 2024 · genes were obtained from the Chlamydomonas Stock Center ( Li et al., 2016b ): (1) TCP10 (T-Complex Protein 10, Cre06.g303200), which was downregulated in LT and northeastern fall online coursesWebThe City of Fawn Creek is located in the State of Kansas. Find directions to Fawn Creek, browse local businesses, landmarks, get current traffic estimates, road conditions, and … northeastern feesWebFeb 1, 2010 · The wild-type C. reinhardtii strain CC-125 (mt+) was obtained from the Chlamydomonas Stock Center (Duke University, Durham, NC, USA). The C. reinhardtii clone CR-463/4 was generated by stably transforming CC-125 (see below) with p463 (5′ rbcL-aadA- 3′ rbcL ) followed by clonal selection using 100 μg/ml spectinomycin. northeastern family and friends weekendWebWelcome to the Chlamydomonas Resource Center, a central repository to receive, catalog, preserve, and distribute high-quality and reliable wild type and mutant cultures of the … Community - Home - Chlamydomonas Resource Center About CRC - Home - Chlamydomonas Resource Center Click the link above for a quick guide to nomenclature rules . This page presents … Genome Browsers - Home - Chlamydomonas Resource Center Databases - Home - Chlamydomonas Resource Center Tools - Home - Chlamydomonas Resource Center Maps - Home - Chlamydomonas Resource Center In the Chlamy Center, we use 150 µ g/ml ampicillin, 50 µ g/ml kanamycin, 50 µ … northeastern family institute vt