Webrepressor, cIts857, to prevent cell killing. Heat induc- tion of the pL-galR fusion plasmid synthesizes large amounts of active Gal repressor. The protein has been purified to homogeneity in three steps. The purifica- tion is greatly aided by the reversible insolubility of active repressor in crude extract at salt concentrations Webcits857。在低温(30℃)时，cits857阻遏蛋白可阻遏pl启动子转录。在高温(45℃)时，cits857蛋白失活，阻遏解除，促使pl启动子转录。系统由于受cits857作用，尤其适合于表达对大肠杆菌有毒的基因产物，缺点是温度转换不仅可诱导pl启动子，也可诱导热休克基 …

Screening of carcinogens with the prophage lambda cIts857 …

WebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble … Web侯云德，男，汉族，1929年7月13日出生于江苏省常州市，中国工程院院士，医学病毒学专家，中国疾病预防控制中心病毒病预防控制所院士实验室主任。1948年至1955年，侯云德本科就读于同济大学医学院；1956年至1957年，侯云德就读于北京俄语学院；1962年至1973年，任中国医学科学院病毒学研究所肝炎 ... flannel network config

原核表达载体的重要调控元件-上海中洪博元

WebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble carcinogens that have been dissolved in dimethyl sulfoxide and metabolically activated with liver enzymes induced either with Aroclor 1254 or phenobarbital. Growth of noninduced ... WebThe galR gene, which encodes the Gal repressor protein in Escherichia coli, has been fused to the strong pL promoter of bacteriophage lambda in plasmid pKC31. The pL promoter is kept repressed by a thermolabilie lambda repressor, CIts857, to prevent cell killing. Heat induction of the pL-galR fusion plasmid synthesizes large amounts of active Gal … flannel native american shirt