Web29 mai 2024 · The planarian flatworm is an important and highly tractable model system for regeneration, stem cell biology, and neuroscience; however, no protocols have been published for investigating pH in this system. Here, we report a simple and effective protocol for imaging pH gradients in living planaria suitable for intact and regenerating flatworms. Web21 feb. 2024 · N 6 -methyladenosine (m6A) is the most prevalent, abundant and conserved internal cotranscriptional modification in eukaryotic RNAs, especially within higher eukaryotic cells. m6A modification is modified by the m6A methyltransferases, or writers, such as METTL3/14/16, RBM15/15B, ZC3H3, VIRMA, CBLL1, WTAP, and KIAA1429, and, …
m6A is required for resolving progenitor identity during planarian …
WebHere, we demonstrate that the depletion of m6A methyltransferase regulatory subunit wtap abolishes planarian regeneration, through regulating cell-cell communication and cell cycle. scRNA-Seq analysis unveils that the wtap knockdown induces a unique type of neural progenitor-like cells (NP-like cells), characterized by specific expression of ... Web15 sept. 2024 · Planarian flatworms are well-known for their regenerative ability, which is dependent on a large population of adult stem cells, called neoblasts, at least some of which are pluripotent. Here,... daily serving of greens
Expression of m6A genes is required for planarian regeneration …
Web15 apr. 2014 · Regeneration in planarians depends on the presence of stem cells called neoblasts. These cells are distributed throughout the body and, when part of the worm has been amputated, they are activated to reform the tissues that have been removed ( Wagner et al., 2011 ). It is still not entirely clear how the stem cells regenerate specific organs. Web16 aug. 2024 · Here, we find that the ubiquitous mRNA base-modification, m6A, is required for proper cell fate choice and cellular maturation in planarian stem cells (neoblasts). We … Webm6A is installed by the m6A methyltransferases complex, which is a dynamical and reversible biological process (Table 1 ). As the first discovered methyltransferase and the … biomes refer to: